Rhodospirillaceae Medium

[所属分类:培养基配方] [发布时间:2021-8-5] [发布人:网站管理员2] [阅读次数:] [返回]

Rhodospirillaceae Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Succinic acid.................................................................................1.0g
KH 2 PO 4 ........................................................................................0.5g
NaCl..............................................................................................0.4g
NH 4 Cl ...........................................................................................0.4g
MgSO 4 ·7H 2 O................................................................................0.2g
Yeast extract..................................................................................0.2g
CaCl 2 ·2H 2 O................................................................................0.05g
Ferric citrate solution.................................................................5.0mL
Vitamin B 12 solution..................................................................1.0mL
Trace elements solution SL7......................................................1.0mL
pH 6.8 ± 0.2 at 25°C
Ferric Citrate Solution:
Composition per 100.0mL:
Ferric citrate..................................................................................0.1g
Preparation of Ferric Citrate Solution: Add ferric citrate to dis-
tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.
Filter sterilize.
Vitamin B 12 Solution:
Composition per 100.0mL:
Vitamin B 12 ................................................................................1.0mg
Preparation of Vitamin B 12 Solution: Add vitamin B 12 to dis-
tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.
Filter sterilize.
Trace Elements Solution SL-7:
Composition per liter:
MnCl 2 ·4H 2 O..........................................................................100.0mg
ZnCl.........................................................................................70.0mg
H 3 Bo 3 .......................................................................................60.0mg
NaMoO 4 ·2H 2 O........................................................................40.0mg
CoCl 2 ·2H 2 O.............................................................................20.0mg
CuCl 2 ·2H 2 O.............................................................................20.0mg
NiCl 2 ·6H 2 O..............................................................................20.0mg
HCl (25%)..................................................................................1.0mL
Preparation of Trace Elements Solution SL7: Add 100mg of
MnCl 2 ·4H 2 O to 1.0mL of HCl solution. Mix thoroughly. Add distilled/
deionized water and bring volume to 1.0L. Add remaining compo-
nents. Mix thoroughly. Sparge with 100% N 2 . Autoclave for 15 min at
15 psi pressure–121°C.
Preparation of Medium: Add components, except ferric -citrate
solution, vitamin B 12 solution, and trace elements solution SL-7, to dis-
tilled/deionized water and bring volume to 993.0mL. Mix thoroughly.
Adjust pH to 6.8. Autoclave for 30 min at 15 psi pressure–121°C.
Aseptically add 5.0mL of sterile ferric citrate solution, 1.0mL of sterile
vitamin B 12 solution, and 1.0mL of sterile trace elements solution SL-
7. Mix thoroughly. Aseptically distribute into sterile screw-capped
tubes under anaerobic conditions. Tighten screw caps.
Use: For the cultivation of Rhodospirillum species and other members
of the family Rhodospirillaceae.