Rhodospirillaceae Enrichment Medium

[所属分类:培养基配方] [发布时间:2021-8-5] [发布人:网站管理员2] [阅读次数:] [返回]

Rhodospirillaceae Enrichment Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Fatty acid substrate.......................................................................1.0g
KH 2 PO 4 .........................................................................................0.5g
NaCl..............................................................................................0.4g
NH 4 Cl ...........................................................................................0.4g
MgSO 4 ·7H 2 O................................................................................0.2g
Yeast extract..................................................................................0.2g
CaCl 2 ·2H 2 O ................................................................................0.05g
NaHCO 3 solution.....................................................................40.0mL
Ferric citrate solution.................................................................5.0mL
Trace elements solution SL-7....................................................1.0mL
Vitamin B 12 solution..................................................................1.0mL
pH 7.3 ± 0.2 at 25°C
Ferric Citrate Solution:
Composition per 100.0mL:
Ferric citrate..................................................................................0.1g
Preparation of Ferric Citrate Solution:  Add ferric citrate to dis-
tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.
Trace Elements Solution SL-7:
Composition per liter:
CoCl 2 ·6H 2 O ..................................................................................0.2g
MnCl 2 ·4H 2 O..................................................................................0.1g
ZnCl 2 ...........................................................................................0.07g
H 3 BO 3 .........................................................................................0.06g
NaMoO 4 ·2H 2 O............................................................................0.04g
CuCl 2 ·2H 2 O ................................................................................0.02g
NiCl 2 ·6H 2 O.................................................................................0.02g
HCl (25% solution)....................................................................1.0mL
Preparation of Trace Elements Solution SL-7:  Add compo-
nents to distilled/deionized water and bring volume to 1.0L. Mix thor-
oughly.
Vitamin B 12 Solution:
Composition per 100.0mL:
Vitamin B 12 ................................................................................1.0mg
Preparation of Vitamin B 12 Solution: Add vitamin B 12 to dis-
tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.
NaHCO 3 Solution:
Composition per 100.0mL:
NaHCO 3 ........................................................................................5.0g
Preparation of NaHCO 3 Solution: Add NaHCO 3 to distilled/de-
ionized water and bring volume to 100.0mL. Mix thoroughly. Filter
sterilize.
Preparation of Medium: Add components, except NaHCO 3 solu-
tion, to distilled/deionized water and bring volume to 1.0L. Acetate,
propionate, or butyrate salts may be used for the fatty acid substrate.
Mix thoroughly. Adjust pH to 7.3. Distribute into flasks in 50.0mL vol-
umes. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C.
Immediately prior to inoculation, aseptically add 2.0mL of sterile
NaHCO 3 solution to each flask containing 50.0mL of medium.
Use: For the enrichment and isolation of members of the Rhodospiril-
laceae.