Rhodopseudomonas Medium (ATCC Medium 650)

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Rhodopseudomonas Medium
(ATCC Medium 650)

山东拓普生物工程有限公司  http://www.topbiol.com

Composition per liter:

Sodium succinate..........................................................................1.5g
KH 2 PO 4 .........................................................................................1.0g
NH 4 Cl ...........................................................................................0.5g
MgSO 4 ·7H 2 O................................................................................0.4g
NaCl..............................................................................................0.4g
CaCl 2 ·2H 2 O.................................................................................0.05g
Trace metals solution...............................................................10.0mL
pH 5.6–6.0 at 25°C
Trace Metals Solution:
Composition per 100.0mL:
Ferric citrate..................................................................................0.3g
Ethylenediamine tetraacetic acid (EDTA)..................................0.05g
CaCl 2 ·2H 2 O ................................................................................0.02g
MnSO 4 ·H 2 O..............................................................................0.002g
(NH 4 ) 6 Mo 7 O 24 ·4H 2 O................................................................0.002g
H 3 BO 3 .......................................................................................0.001g
CuSO 4 ·5H 2 O.............................................................................0.001g
ZnSO 4 .......................................................................................0.001g
Preparation of Trace Metals Solution: Add components to dis-
tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.
Filter sterilize.
Preparation of Medium: Add components, except trace metals so-
lution, to distilled/deionized water and bring volume to 990.0mL. Mix
thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to
25°C. Aseptically add 10.0mL of trace metals solution. Mix thorough-
ly. Aseptically distribute into sterile tubes or flasks.
Use:  For the cultivation and maintenance of Rhodopseudomonas
viridis, Rhodopseudomonas acidophila, and other Rhodopseudomonas
species.