Rhodocyclus Medium (LMG Medium 82)

[所属分类:培养基配方] [发布时间:2021-8-4] [发布人:网站管理员2] [阅读次数:] [返回]

Rhodocyclus Medium
(LMG Medium 82)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Yeast extract..................................................................................1.0g
Ammonium acetate.......................................................................0.5g
KH 2 PO 4 .........................................................................................0.5g
MgSO 4 ·7H2O ...............................................................................0.4g
NaCl..............................................................................................0.4g
NH 4 Cl ...........................................................................................0.4g
CaCl 2 ·2H 2 O .............................................................................50.0mg
Ferric citrate solution.................................................................5.0mL
Trace elements solution.............................................................1.0mL
Vitamin solution.........................................................................1.0mL
Ethanol.......................................................................................0.5mL
pH 5.8 ± 0.2 at 25°C
Ferric Citrate Solution :
Composition per 100.0mL:
Ferric citrate..................................................................................0.1g
Preparation of Ferric Citrate Solution:  Add ferric citrate to dis-
tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.
Trace Elements Solution:
Composition per liter:
H 3 BO 3 ...........................................................................................0.3g
CoCl 2 ·6H 2 O..................................................................................0.2g
ZnSO 4 ·7H 2 O.................................................................................0.1g
Na 2 MoO 4 ·2H 2 O.......................................................................30.0mg
MnCl 2 ·4H 2 O ............................................................................30.0mg
NiCl 2 ·6H 2 O..............................................................................20.0mg
CuCl 2 ·2H 2 O .............................................................................10.0mg
Preparation of Trace Elements Solution:  Add components to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Vitamin Solution:
Composition per 100.0mL:
Vitamin B 12 ................................................................................2.0mg
Preparation of Vitamin Solution: Add vitamin B 12 to 100.0mL of
distilled/deionized water. Mix thoroughly.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute 40.0mL
medium into 50 mL screw-capped bottles. Flush each bottle for 1 to 2
minutes with nitrogen gas and then close immediately with rubber sep-
ta and screw caps. Autoclave for 15 min at 15 psi pressure–121°C.
Sterile syringes are used to inoculate and remove the samples. Incubate
in light using a tungsten lamp.
Use: For the cultivation of Rhodocyclus purpureus.