Rhodobium gokurnum Medium (DSMZ Medium 1129)

[所属分类:培养基配方] [发布时间:2021-8-4] [发布人:网站管理员2] [阅读次数:] [返回]

Rhodobium gokurnum Medium
(DSMZ Medium 1129)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NaCl............................................................................................20.0g
MgCl 2 · 6H 2 O .................................................................................1.0g
KH 2 PO 4 .........................................................................................0.5g
NH 4 Cl ........................................................................................0.34g
CaC l 2 ·2H 2 O ................................................................................0.15g
Sulfide solution........................................................................10.0mL
Thiosulfate solution.................................................................10.0mL
Bicarbonate solution................................................................10.0mL
Yeast extract solution...............................................................10.0mL
Sodium pyruvate solution........................................................10.0mL
Ferric citrate solution.................................................................5.0mL
Trace elements solution SL-7....................................................1.0mL
Vitamin solution.........................................................................1.0mL
pH 7.2 ± 0.2 at 25°C
Ferric Citrate Solution :
Composition per 10.0mL:
Ferric citrate................................................................................0.01g
Preparation of Ferric Citrate Solution:  Add ferric citrate to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Filter sterilize.
Trace Elements Solution SL-7:
Composition per 1001.0mL:
CoCl 2 ·6H 2 O ...........................................................................200.0mg
MnCl 2 ·4H 2 O...........................................................................100.0mg
ZnCl 2 ........................................................................................70.0mg
H 3 BO 3 ......................................................................................60.0mg
Na 2 MoO 4 ·2H 2 O .......................................................................40.0mg
CuCl 2 ·2H 2 O .............................................................................20.0mg
NiCl 2 ·6H 2 O..............................................................................20.0mg
HCl (25%)..................................................................................1.0mL
Preparation of Trace Elements Solution SL-7:  Add components
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Vitamin Solution:
Composition per liter:
Vitamin B 12 ................................................................................2.0mg
Preparation of Vitamin Solution:  Add vitamin B 12 to distilled/
deionized water and bring volume to 1.0L. Mix thoroughly. Filter ster-
ilize.
Sodium Pyruvate Solution:
Composition per 10.0mL:
Sodium pyruvate...........................................................................3.0g
Preparation of Sodium Pyruvate Solution:  Add sodium pyru-
vate to distilled/deionized water and bring volume to 10.0mL. Mix
thoroughly. Filter sterilize.
Yeast Extract Solution:
Composition per 10.0mL:
Yeast extract..................................................................................0.4g
Preparation of Yeast Extract Solution:  Add yeast extract to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Filter sterilize.
Sulfide Solution :
Composition per 10.0mL:
Na 2 S·9H 2 O..................................................................................0.25g
Preparation of Sulfide Solution: Add Na 2 S·9H 2 O to distilled/de-
ionized water and bring volume to 10.0mL. Mix thoroughly. Autoclave
under 100% N 2 for 15 min at 15 psi pressure–121°C. Cool to room
temperature.
Thiosulfate Solution:
Composition per 10.0mL:
Na 2 S 2 O 3 ·5H 2 O..............................................................................1.6g
Preparation of Thiosulfate Solution:  Add Na 2 S 2 O 3 ·5H 2 O to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Sparge with 100% N 2 . Adjust to pH 10.0. Filter sterilize.
Bicarbonate Solution :
Composition per 100.0mL:
NaHCO 3 ......................................................................................10.0g
Preparation of Bicarbonate Solution:  Add NaHCO 3 to distilled/
deionized water and bring volume to 100.0mL. Mix thoroughly. Adjust
pH to 6.5. Seal in bottles (half full) under an atmosphere of CO 2 . Au-
toclave for 15 min at 15 psi pressure–121°C. Cool to room tempera-
ture.
Preparation of Medium: Add components, except ferric citrate,
trace elements, sulfide, thiosulfate, bicarbonate, vitamin, sodium pyru-
vate, and yeast extract solutions, to distilled/deionized water and bring
volume to 943.0mL. Mix thoroughly. Adjust pH to 7.2. Gently heat and
bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool
to room temperature. Aseptically add ferric citrate, trace elements, sul-
fide, thiosulfate, bicarbonate, vitamin, sodium pyruvate, and yeast ex-
tract solutions. Mix thoroughly. Aseptically distribute into culture
vessels.
Use: For the cultivation of Allochromatium renukae and Allochroma-
tium roseum.