Rhodobium gokurnum Medium (DSMZ Medium 1129)

[所属分类:培养基配方] [发布时间:2021-8-4] [发布人:网站管理员2] [阅读次数:] [返回]

Rhodobium gokurnum Medium
(DSMZ Medium 1129)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NaCl............................................................................................20.0g
KH 2 PO 4 .........................................................................................0.5g
MgCl 2 · 6H 2 O..................................................................................1.0g
NH 4 Cl ..........................................................................................0.6g
CaC l 2 ·2H 2 O ................................................................................0.15g
Sorbitol solution.......................................................................10.0mL
Yeast extract solution...............................................................10.0mL
Sodium pyruvate solution........................................................10.0mL
Ferric citrate solution.................................................................5.0mL
Trace elements solution SL-7 ....................................................1.0mL
pH 6.5 ± 0.2 at 25°C
Ferric Citrate Solution :
Composition per 10.0mL:
Ferric citrate................................................................................0.01g
Preparation of Ferric Citrate Solution:  Add ferric citrate to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Filter sterilize.
Trace Elements Solution SL-7:
Composition per liter:
CoCl 2 ·6H 2 O ...........................................................................200.0mg
MnCl 2 ·4H 2 O...........................................................................100.0mg
ZnCl 2 ........................................................................................70.0mg
H 3 BO 3 ......................................................................................60.0mg
Na 2 MoO 4 ·2H 2 O .......................................................................40.0mg
CuCl 2 ·2H 2 O .............................................................................20.0mg
NiCl 2 ·6H 2 O..............................................................................20.0mg
HCl (25%)..................................................................................1.0mL
Preparation of Trace Elements Solution SL-7:  Add components
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Sorbitol Solution:
Composition per 10.0mL:
Sorbitol..........................................................................................3.0g
Preparation of Sorbitol Solution:  Add sorbitol to distilled/deion-
ized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.
Sodium Pyruvate Solution:
Composition per 10.0mL:
Sodium pyruvate...........................................................................3.0g
Preparation of Sodium Pyruvate Solution:  Add sodium pyru-
vate to distilled/deionized water and bring volume to 10.0mL. Mix
thoroughly. Filter sterilize.
Yeast Extract Solution:
Composition per 10.0mL:
Yeast extract..................................................................................0.4g
Preparation of Yeast Extract Solution:  Add yeast extract to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Filter sterilize.
Preparation of Medium: Add components, except ferric citrate,
trace elements, sorbitol, sodium pyruvate, and yeast extract solutions,
to distilled/deionized water and bring volume to 964.0mL. Mix thor-
oughly. Adjust pH to 6.5. Gently heat and bring to boiling. Autoclave
for 15 min at 15 psi pressure–121°C.Cool to room temperature. Asep-
tically add ferric citrate, trace elements, sorbitol, sodium pyruvate, and
yeast extract solutions. Mix thoroughly. Aseptically distribute into cul-
ture vessels.
Use: For the cultivation of Rhodobium gokarnense (Rhodobium
gokurnum).