Rhodobacter adriaticus Medium

[所属分类:培养基配方] [发布时间:2021-8-4] [发布人:网站管理员2] [阅读次数:] [返回]

Rhodobacter adriaticus Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 1001.0mL:

NaCl............................................................................................25.0g
NaHCO 3 ........................................................................................3.0g
K 2 HPO 4 .........................................................................................1.0g
NH 4 Cl ...........................................................................................1.0g
MgCl 2 ·6H 2 O..................................................................................0.5g
Sodium ascorbate..........................................................................0.5g
CaCl 2 ·2H 2 O...................................................................................0.1g
Trace elements solution SLA.....................................................1.0mL
Vitamin solution.........................................................................1.0mL
pH 7.0 ± 0.2 at 25°C
Trace Elements Solution SLA:
Composition per liter:
CuCl 2 ·2H 2 O ................................................................................10.0g
FeCl 2 ·4H 2 O...................................................................................1.8g
H 3 BO 3 ...........................................................................................0.5g
CoCl 2 ·6H 2 O ................................................................................0.25g
ZnCl 2 .............................................................................................0.1g
MnCl 2 ·4H 2 O.............................................................................70.0mg
Na 2 MoO 4 ·2H 2 O .......................................................................30.0mg
Na 2 SeO 3 ·5H 2 O.........................................................................10.0mg
NiCl 2 ·6H 2 O..............................................................................10.0mg
Preparation of Trace Elements Solution SLA : Add compo-
nents to distilled/deionized water and bring volume to 1.0L. Mix thor-
oughly. Bring pH to 2.0–3.0.
Vitamin Solution:
Composition per liter:
Nicotinamide............................................................................35.0mg
Thiamine·HCl ..........................................................................30.0mg
p-Aminobenzoic acid...............................................................20.0mg
Pyridoxal·HCl..........................................................................10.0mg
Calcium  DL -pantothenate.........................................................10.0mg
Biotin .......................................................................................10.0mg
Vitamin B 12 ................................................................................5.0mg
Preparation of Vitamin Solution : Add components to distilled/
deionized water and bring volume to 1.0L. Mix thoroughly. Filter ster-
ilize.
Preparation of Medium: Add components, except vitamin solu-
tion, to distilled/deionized water and bring volume to 1.0L. Mix thor-
oughly. Autoclave for 15 min at 15 psi pressure–121°C. Aseptically
add 1.0mL of sterile vitamin solution. Mix thoroughly. Aseptically dis-
tribute into sterile tubes or flasks.
Use: For the cultivation and maintenance of Rhodobacter adraiticus.