Regan-Lowe Semisolid Transport Medium

[所属分类:培养基配方] [发布时间:2021-8-3] [发布人:网站管理员2] [阅读次数:] [返回]

Regan-Lowe Semisolid Transport Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar..............................................................................................6.0g
Beef extract...................................................................................5.0g
Pancreatic digest of gelatin...........................................................5.0g
Soluble starch................................................................................5.0g
NaCl..............................................................................................2.5g
Charcoal........................................................................................2.0g
Niacin..........................................................................................0.01g
Horse blood, defibrinated ......................................................100.0mL
Cephalexin solution.................................................................10.0mL
pH 7.4 ± 0.2 at 25°C
Cephalexin Solution:
Composition per 10.0mL:
Cephalexin..................................................................................0.04g
Preparation of Cephalexin Solution: Add cephalexin to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Filter sterilize.
Preparation of Medium: Add components, except horse blood and
cephalexin solution, to distilled/deionized water and bring volume to
890.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for
15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add ster-
ile horse blood and sterile cephalexin solution. Mix thoroughly. Aseptical-
ly distribute into small, sterile, screw-capped tubes. Fill tubes half-full.
Swirl medium while dispensing to keep charcoal in suspension.
Use: For the transport of Bordetella pertussis and Bordetella paraper-
tussis isolated from clinical specimens.