Allantoin Broth

[所属分类:培养基配方] [发布时间:2019-9-29] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Allantoin Broth
Composition per liter:
Na 2 HPO 4 ·12H 2 O..........................................................................9.0g
NaCl..............................................................................................5.0g
KH 2 PO 4 ........................................................................................1.5g
Meat extract..................................................................................1.0g
Yeast extract..................................................................................1.0g
MgSO 4 ·7H 2 O................................................................................0.2g
MnCl 2 ·4H 2 O............................................................................20.0mg
CaCl 2 .........................................................................................1.2mg
Glucose-allantoin solution.....................................................100.0mL
Glucose-Allantoin Solution:
Composition per 100.0mL:
Glucose.........................................................................................5.0g
Allantoin.......................................................................................1.0g
Preparation of Glucose-Allantoin Solution:  Add components
to distilled/deionized water and bring volume to 100.0mL. Mix thor-
oughly. Filter sterilize.
Preparation of Medium: Add components, except glucose-allant-
oin solution, to distilled/deionized water and bring volume to
900.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–
121°C. Aseptically add 100.0mL of sterile glucose-allantoin solution.
Mix thoroughly. Aseptically distribute into sterile tubes or flasks.
Use:  For the cultivation and maintenance of Bacillus species.