Alkalophile Medium

[所属分类:培养基配方] [发布时间:2019-9-29] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Alkalophile Medium
Composition per liter:
Agar ............................................................................................15.0g
Peptone..........................................................................................5.0g
NaCl..............................................................................................5.0g
Yeast extract..................................................................................2.0g
Beef extract...................................................................................1.0g
Sodium sesquicarbonate solution.............................................15.0mL
pH 9.5 ± 0.2 at 25°C
Sodium Sesquicarbonate Solution:
Composition per 100.0mL:
Sodium sesquicarbonate ...............................................................9.0g
Preparation of Sodium Sesquicarbonate Solution:  Add sodi-
um sesquicarbonate to distilled/deionized water and bring volume to
100.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except sodium sesqui-
carbonate solution, to distilled/deionized water and bring volume to
985.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave
for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C. Aseptically
add 15.0mL of filter-sterilized sodium sesquicarbonate solution to ad-
just pH to 9.5. Mix thoroughly. Pour into sterile Petri dishes or distrib-
ute into sterile tubes.
Use: For the cultivation of Bacillus alcalophilus, Bacillus circulans,
Bacillus submarinus, and other Bacillus species.