Alkaliphilic Sulphur Respiring Strains Medium (DSMZ Medium 925)

[所属分类:培养基配方] [发布时间:2019-9-27] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Alkaliphilic Sulphur Respiring Strains Medium (DSMZ Medium 925)
Composition per liter:
Mineral base...........................................................................967.5mL
Thiosulfate solution .................................................................20.0mL
KSCN solution.........................................................................10.0mL
Trace elements solution .............................................................2.0mL
Magnesium chloride solution.....................................................0.5mL
pH 10.0 ± 0.2 at 25°C
Mineral Base:
Composition per liter:
Na 2 CO 3 .......................................................................................20.0g
NaHCO 3 ......................................................................................10.0g
NaCl..............................................................................................5.0g
K 2 HPO 4 .........................................................................................1.0g
Preparation of Mineral Base: Add components to distilled/deion-
ized water and bring volume to 1.0L. Mix thoroughly. Sparge with
100% N 2 . Autoclave for 20 min at 110°C. The pH should be about
10.0.
Trace Elements Solution:
Composition per liter:
H 3 BO 3 ....................................................................................300.0mg
CoCl 2 ·6H 2 O ...........................................................................200.0mg
ZnSO 4 ·7H 2 O..........................................................................100.0mg
MnCl 2 ·4H 2 O.............................................................................30.0mg
Na 2 MoO 4 ·4H 2 O .......................................................................30.0mg
NiCl 2 ·6H 2 O..............................................................................20.0mg
CuCl 2 ·2H 2 O .............................................................................10.0mg
EDTA.........................................................................................5.0mg
FeSO 4 ·7H 2 O...............................................................................2.0mg
Preparation of Trace Elements Solution: Add components to
distilled/deionized water and bring volume to 1.0L. Adjust pH to 3.0
with HCl. Mix thoroughly. Sparge with 100% N 2 . Autoclave for 15 min
at 15 psi pressure–121°C.
Magnesium Chloride Solution:
Composition per 10.0mL:
MgCl 2 ·6H 2 O..................................................................................2.0g
Preparation of Magnesium Chloride Solution: Add
MgCl 2 ·6H 2 O to distilled/deionized water and bring volume to 10.0mL.
Mix thoroughly. A white colloid will dissolve after mixing. Autoclave
for 15 min at 15 psi pressure–121°C.
Thiosulfate Solution:
Composition per liter:
Na 2 S 2 O 3 ·5H 2 O............................................................................9.92g
Preparation of Thiosulfate Solution:  Add Na 2 S 2 O 3 ·5H 2 O to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly. Au-
toclave for 15 min at 15 psi pressure–121°C. Cool to room
temperature.
KSCN Solution:
Composition per 10.0mL:
KSCN............................................................................................0.5g
Preparation of KSCN Solution:  Add KSCN to distilled/deionized
water and bring volume to 10.0mL. Mix thoroughly. Autoclave for 15
min at 15 psi pressure–121°C. Cool to room temperature.
Preparation of Medium: Aseptically add 20.0mL sterile thiosul-
fate solution, 10.0mL sterile KSCN solution, 2.0mL sterile trace ele-
ments solution, and 0.5mL sterile magnesium chloride solution to
967.5mL sterile mineral base. Aseptically distribute to sterile tubes,
flasks, or bottles.
Use: For the cultivation of Thioalkalivibrio versutus DSM 13738, Thio-
alkalivibrio versutus DSM 13741, and Thioalkalivibrio denitrificans.