Alkaline Xylan Broth

[所属分类:培养基配方] [发布时间:2019-9-26] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Alkaline Xylan Broth
Composition per liter:
Xylan...........................................................................................10.0g
Yeast extract..................................................................................3.0g
NH 4 NO 3 ........................................................................................2.0g
K 2 HPO 4 .........................................................................................1.0g
Polypepton.............................................................................300.0mg
MgSO 4 ·7H 2 O.........................................................................200.0mg
CaCl 2 ·2H 2 O ...........................................................................100.0mg
FeSO 4 ·7H 2 O...............................................................................5.0mg
MnSO 4 ·7H 2 O.............................................................................5.0mg
Resazurin ...................................................................................1.0mg
pH 10.0 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Autoclave for 15 min
at 15 psi pressure–121°C. Aseptically adjust pH with 10% sterile
Na 2 S 2 O 3 solution. Mix thoroughly. Aseptically distribute into sterile
tubes or flasks.
Use: For the cultivation of Amphibacillus xylanus.