Peptone Water with Andrade’s Indicator

[所属分类:培养基配方] [发布时间:2021-7-2] [发布人:网站管理员2] [阅读次数:] [返回]

Peptone Water with Andrade’s Indicator

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Peptone........................................................................................10.0g
NaCl..............................................................................................5.0g
Andrade’s indicator................................................................100.0mL
Carbohydrate solution..............................................................20.0mL
pH 7.4 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from Oxoid
Unipath.
Andrade’s Indicator:
Composition per 100.0mL:
NaOH (1N solution).................................................................16.0mL
Acid Fuchsin................................................................................0.1 g
Preparation of Andrade’s Indicator: Add Acid Fuchsin to
NaOH solution and bring volume to 100.0mL with distilled/deionized
water.
Caution: Acid Fuchsin is a potential carcinogen and care must be tak-
en to avoid inhalation of the powdered dye and contact with the skin.
Carbohydrate Solution:
Composition per 20.0mL:
Carbohydrate........................................................................5.0–10.0g
Preparation of Carbohydrate Solution: Add carbohydrate to
distilled/deionized water and bring volume to 20.0mL. Mix thorough-
ly. Filter sterilize.
Preparation of Medium: Add components, except carbohydrate
solution, to distilled/deionized water and bring volume to 980.0mL.
Mix thoroughly. Adjust pH to 7.4 if necessary. Distribute into tubes
containing an inverted Durham tube. Fill each tube with 9.8mL of me-
dium. Autoclave for 15 min at 15 psi pressure–121°C. Aseptically add
0.2mL of sterile carbohydrate solution to each tube.
Use: For use in carbohydrate fermentation tests. Fermentation is deter-
mined by the production of acid—broth turns pink—and formation of
gas—bubble trapped in Durham tube.