Peptone Starch Dextrose Agar (PSD Agar) (Dunkelberg Agar)

[所属分类:培养基配方] [发布时间:2021-7-2] [发布人:网站管理员2] [阅读次数:] [返回]

Peptone Starch Dextrose Agar
(PSD Agar)
(Dunkelberg Agar)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Proteose peptone No. 3...............................................................20.0g
Agar............................................................................................15.0g
Soluble starch..............................................................................10.0g
Glucose.........................................................................................2.0g
Na 2 HPO 4 .......................................................................................1.0g
NaH 2 PO 4 .......................................................................................1.0g
pH 6.8 ± 0.2 at 25°C
Preparation of Medium: Add starch to approximately 100.0mL of
cold distilled/deionized water. Mix thoroughly. Add starch solution to
400.0mL of boiling distilled/deionized water. Add remaining compo-
nents. Mix thoroughly. Bring volume to 1.0L with distilled/deionized
water. Autoclave for 12 min at 8 psi pressure–112°C. Pour into sterile
Petri dishes or distribute into screw-capped tubes.
Use:  For the selective isolation and cultivation of Gardnerella vagina-
lis.