Alkaline Starch Agar

[所属分类:培养基配方] [发布时间:2019-9-26] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Alkaline Starch Agar
Composition per liter:
Starch..........................................................................................20.0g
Agar............................................................................................16.0g
Na 2 CO 3 .......................................................................................10.0g
Peptone .........................................................................................6.0g
Yeast extract..................................................................................3.0g
K 2 HPO 4 .........................................................................................1.0g
MgSO 4 ·7H 2 O................................................................................0.2g
MnSO 4 .....................................................................................40.0mg
pH 9.7 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Bring pH to 9.7. Gen-
tly heat and bring to boiling. Distribute into tubes or flasks. Autoclave
for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or
leave in tubes.
Use: For the cultivation of alkiliniphilic starch-utilizing bacteria.