Novobiocin Agar

[所属分类:培养基配方] [发布时间:2021-6-21] [发布人:网站管理员2] [阅读次数:] [返回]

Novobiocin Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar............................................................................................15.0g
Peptone .........................................................................................5.0g
NaCl..............................................................................................5.0g
Yeast extract..................................................................................2.0g
Beef extract...................................................................................1.0g
Novobiocin solution.................................................................10.0mL
Novobiocin Solution:
Composition per 10.0mL:
Novobiocin ..............................................................................10.0mg
Preparation of Novobiocin Solution: Add novobiocin to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Filter sterilize.
Preparation of Medium: Add components, except novobiocin so-
lution, to distilled/deionized water and bring volume to 990.0mL. Mix
thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at
15 psi pressure–121°C. Aseptically add 10.0mL of sterile novobiocin
solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into
sterile tubes.
Use: For the cultivation of Staphylococcus aureus.