Nonnutrient Agar Plates

[所属分类:培养基配方] [发布时间:2021-6-21] [发布人:网站管理员2] [阅读次数:] [返回]

Nonnutrient Agar Plates

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar............................................................................................15.0g
Page’s amoeba saline....................................................................1.0L
Page’s Amoeba Saline:
Composition per liter:
Na 2 HPO 4 ...................................................................................0.142g
KH 2 PO 4 .....................................................................................0.136g
NaCl............................................................................................0.12g
MgSO 4 ·7H 2 O.............................................................................4.0mg
CaCl 2 ·2H 2 O ...............................................................................4.0mg
Preparation of Page’s Amoeba Saline: Add components to dis-
tilled/deionized water and bring volume to 1.0mL. Mix thoroughly.
Preparation of Medium: Add agar to 1.0L of Page’s amoeba saline.
Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at
15 psi pressure–121°C. Cool to 60°C. Pour into sterile Petri dishes in
20.0mL volumes. Store at 4°C for up to 3 months.
Use: For the isolation and cultivation of pathogenic free-living amoe-
bae.