Nocardia Medium 1

[所属分类:培养基配方] [发布时间:2021-6-21] [发布人:网站管理员2] [阅读次数:] [返回]

Nocardia Medium 1

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 1010.0mL:

Agar ............................................................................................12.0g
Proteose peptone.........................................................................10.0g
Veal infusion solids.....................................................................10.0g
NaCl..............................................................................................3.0g
Na 2 HPO 4 .......................................................................................2.0g
Glucose .........................................................................................2.0g
Sodium acetate..............................................................................1.0g
Adenine sulfate...........................................................................0.01g
Guanine·HCl...............................................................................0.01g
Uracil ..........................................................................................0.01g
Xanthine......................................................................................0.01g
Thiamine..................................................................................0.02mg
Additives solution....................................................................10.0mL
pH 7.4 ± 0.2 at 25°C
Additives Solution:
Composition per 10.0mL:
Actidione (cycloheximide) ......................................................0.05mg
Mycostatin ...............................................................................0.05mg
Dimethylchlortetracycline·HCl...................................................5.0μg
Preparation of Additives Solution: Add components to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Filter
sterilize.
Caution:  Cycloheximide is toxic. Avoid skin contact or aerosol for-
mation and inhalation.
Preparation of Medium: Add components, except additives solu-
tion, to distilled/deionized water and bring volume to 990.0mL. Mix
thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at
15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add additives
solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into
sterile tubes.
Use: For the isolation and cultivation of Nocardia.