NNN Medium (Novy, MacNeal, and Nicole Medium)

[所属分类:培养基配方] [发布时间:2021-6-21] [发布人:网站管理员2] [阅读次数:] [返回]

NNN Medium
(Novy, MacNeal, and Nicole Medium)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar..............................................................................................7.0g
NaCl..............................................................................................3.0g
Rabbit blood, defibrinated.....................................................150.0mL
Preparation of Medium: Add components, except rabbit blood, to
distilled/deionized water and bring volume to 850.0mL. Mix thorough-
ly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 50°C. Aseptically add sterile rabbit blood.
Mix thoroughly. Aseptically distribute into sterile tubes in 5.0mL vol-
umes. Allow tubes to cool in a slanted position at 4°C.
Use: For the cultivation and maintenance of Leishmania species and
Trypanosoma cruzi.