NMS Medium

[所属分类:培养基配方] [发布时间:2021-6-21] [发布人:网站管理员2] [阅读次数:] [返回]

NMS Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 1000.5mL:

Purified agar................................................................................12.5g
KNO 3 ............................................................................................1.0g
MgSO 4 ·7H 2 O................................................................................1.0g
Na 2 HPO 4 ·12H 2 O.......................................................................0.717g
KH 2 PO 4 .....................................................................................0.272g
CaCl 2 ·6H 2 O...................................................................................0.2g
Ferric ammonium EDTA ...........................................................4.0mg
Trace elements solution .............................................................0.5mL
pH 6.8 ± 0.2 at 25°C
Trace Elements Solution:
Composition per liter:
Disodium EDTA ...........................................................................0.5g
FeSO 4 ·7H 2 O..................................................................................0.2g
H 3 BO 3 ......................................................................................30.0mg
CoCl 2 ·6H 2 O .............................................................................20.0mg
ZnSO 4 ·7H 2 O............................................................................10.0mg
MnCl 2 ·4H 2 O...............................................................................3.0mg
Na 2 MoO 4 ·2H 2 O .........................................................................3.0mg
NiCl 2 ·6H 2 O................................................................................2.0mg
CaCl 2 ·2H 2 O................................................................................1.0mg
Preparation of Trace Elements Solution : Add components to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Autoclave for 15 min at 15 psi pressure–121°C.
Preparation of Medium: Add components, except trace elements
solution, to distilled/deionized water and bring volume to 1.0L. Mix
thoroughly. Adjust pH to 6.8. Autoclave for 15 min at 15 psi pressure–
121°C. Aseptically add 0.5mL of sterile trace elements solution. Mix
thoroughly. Aseptically distribute into sterile tubes or flasks.
Use:  For the cultivation of Methylomonas clara.