Alicyclobacillus ferrooxydans Medium (DSMZ Medium 1201)

[所属分类:培养基配方] [发布时间:2019-9-24] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Alicyclobacillus ferrooxydans Medium
(DSMZ Medium 1201)
Composition per liter:
MgSO 4 ·7H 2 O ....................................................................... 0.5g
(NH 4 ) 2 SO 4 ........................................................................... 0.4g
K 2 HPO 4 ........................................................................................ 0.2g
Yeast extract..................................................................................0.2g
K 2 S 4 O 6 ........................................................................................0.15g
KCl................................................................................................0.1g
MnSO 4 ·H 2 O ........................................................................0.01g
Iron sulfate solution.................................................................70.0mL
pH 1.8–2.5 at 25°C
Iron Sulfate Solution:
Composition per 100.0mL:
FeSO 4 ·7H 2 O................................................................................20.0g
Preparation of Iron Sulfate Solution:  Add components to 0.2N
H 2 SO 4 and bring volume with distilled/deionized water to 100.0mL.
Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except iron sulfate so-
lution, to distilled/deionized water and bring volume to 970.0mL. Mix
thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at
15 psi pressure–121°C. Cool to 50°C. Adjust pH to approximately 2.2.
Aseptically add 70.0mL of iron sulfate solution. Mix thoroughly. The
pH should be 1.8–2.5.
Use: For the maintenance or cultivation of Alicyclobacillus ferrooxy-
dans.