MRS HiVeg Agar, Modified (Lactobacilli Heteroferm Screen HiVeg Agar)

[所属分类:培养基配方] [发布时间:2021-6-8] [发布人:网站管理员2] [阅读次数:] [返回]

MRS HiVeg Agar, Modified
(Lactobacilli Heteroferm Screen HiVeg Agar)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Glucose.......................................................................................20.0g
Agar............................................................................................15.0g
Plant peptone No. 3.....................................................................10.0g
Sodium acetate..............................................................................5.0g
Yeast extract..................................................................................5.0g
2-Phenylethyl alcohol...................................................................3.0g
Ammonium citrate........................................................................2.0g
K 2 HPO 4 .........................................................................................2.0g
MgSO 4 ..........................................................................................0.1g
MnSO 4 ........................................................................................0.05g
Bromcresol Green.......................................................................0.04g
Cycloheximide...........................................................................4.0mg
pH 6.2 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from Hi-
Media.
Caution:  Cycloheximide is toxic. Avoid skin contact or aerosol for-
mation and inhalation.
Preparation of Medium:  Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the isolation and cultivation of Lactobacillus species from
salad dressings.