Motility Test Medium

[所属分类:培养基配方] [发布时间:2021-6-7] [发布人:网站管理员2] [阅读次数:] [返回]

Motility Test Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of gelatin.........................................................10.0g
NaCl..............................................................................................5.0g
Agar..............................................................................................4.0g
Beef extract...................................................................................3.0g
Triphenyltetrazolium chloride solution .....................................5.0mL
pH 7.3 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
2,3,5-Triphenyltetrazolium Chloride Solution:
Composition per 10.0mL:
2,3,5-Triphenyltetrazolium chloride.............................................0.1g
Preparation of 2,3,5-Triphenyltetrazolium Chloride Solu-
tion: Add 2,3,5-triphenyltetrazolium chloride to distilled/deionized
water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 995.0mL. Mix thoroughly. Gently heat
while stirring and bring to boiling. Autoclave for 15 min at 15 psi pres-
sure–121°C. Cool to 45°–50°C. Aseptically add 5.0mL of sterile 2,3,5-
triphenyltetrazolium chloride solution. Mix thoroughly. Aseptically
distribute into sterile tubes.
Use: For detection of the motility of Gram-negative enteric bacteria.