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Motility Nitrate Medium, Buffered

[所属分类:培养基配方] [发布时间:2021-6-7] [发布人:网站管理员2] [阅读次数:] [返回]
Motility Nitrate Medium, Buffered

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Peptone..........................................................................................5.0g
Galactose.......................................................................................5.0g
Agar ..............................................................................................3.0g
Beef extract...................................................................................3.0g
Na 2 HPO 4 .......................................................................................2.5g
KNO 3 ............................................................................................1.0g
Glycerin .....................................................................................5.0mL
pH 7.3 ± 0.1 at 25°C
Preparation of Medium: Add components, except agar, to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gen-
tly heat until dissolved. Add agar. Gently heat until boiling. Distribute
into tubes in 11.0mL volumes. Autoclave for 15 min at 15 psi pressure–
121°C. If not used within 4 hr, heat tubes to 100°C for 10 min.
Use: For the cultivation and observation of the motility of Clostridium
perfringens.
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