Motility Nitrate HiVeg Medium, Buffered

[所属分类:培养基配方] [发布时间:2021-6-7] [发布人:网站管理员2] [阅读次数:] [返回]

Motility Nitrate HiVeg Medium, Buffered

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Galactose.......................................................................................5.0g
Plant extract ..................................................................................3.0g
Plant peptone.................................................................................5.0g
KNO 3 ............................................................................................5.0g
Agar ..............................................................................................3.0g
Na 2 HPO 4 .......................................................................................2.5g
Glycerol .....................................................................................5.0mL

pH 7.4 ± 0.2 at 25°C

Source: This medium, without glycerol, is available as a premixed
powder from HiMedia.
Preparation of Medium: Add glycerol followed by other compo-
nents to distilled/deionized water and bring volume to 1.0L. Mix thor-
oughly. Gently heat and bring to boiling. Distribute into tubes in 4.0mL
volumes. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the cultivation and observation of motility and nitrate reduc-
tion in a variety of Gram-negative bacteria.