Motility Medium S

[所属分类:培养基配方] [发布时间:2021-6-7] [发布人:网站管理员2] [阅读次数:] [返回]

Motility Medium S

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Beef heart, solids from infusion................................................500.0g
Gelatin.........................................................................................30.0g
Enzymatic hydrolyzate of protein...............................................10.0g
NaCl..............................................................................................5.0g
K 2 HPO 4 .........................................................................................2.0g
KNO 3 ............................................................................................2.0g
Agar ..............................................................................................1.0g
2,3,5-Triphenyltetrazolium chloride solution..........................10.0mL
pH 7.2 ± 0.2 at 25°C
2,3,5-Triphenyltetrazolium Chloride Solution:
Composition per 10.0mL:
2,3,5-Triphenyltetrazolium chloride.............................................0.1g
Preparation of 2,3,5-Triphenyltetrazolium Chloride Solu-
tion:  Add 2,3,5-triphenyltetrazolium chloride to distilled/deionized
water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except 2,3,5-triphe-
nyltetrazolium chloride solution, to distilled/deionized water and bring
volume to 990.0mL. Mix thoroughly. Gently heat while stirring and
bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool
to 60°C. Aseptically add 10.0mL of the sterile 2,3,5-triphenyltetrazoli-
um chloride solution. Mix thoroughly. Aseptically distribute into ster-
ile tubes. Keep at 4°–8°C until used.
Use: For the determination of bacterial motility.