Motility Indole Ornithine Medium (MIO Medium)

[所属分类:培养基配方] [发布时间:2021-6-7] [发布人:网站管理员2] [阅读次数:] [返回]

Motility Indole Ornithine Medium
(MIO Medium)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of gelatin.........................................................10.0g
Pancreatic digest of casein............................................................9.5g
L- Ornithine·HCl ............................................................................5.0g
Yeast extract..................................................................................3.0g
Agar..............................................................................................2.0g
Glucose.........................................................................................1.5g
Bromcresol Purple......................................................................0.02g
pH 6.6 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Medium: Add components to distilled/deionized
water and bring to 1.0L. Mix thoroughly. Gently heat and bring to boil-
ing. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pres-
sure–121°C.
Use: For the differentiation of Gram-negative enteric bacteria based
on their motility, indole production, and ornithine decarboxylase activ-
ity.