Moraxella Medium (LMG Medium 204)

[所属分类:培养基配方] [发布时间:2021-6-7] [发布人:网站管理员2] [阅读次数:] [返回]

Moraxella Medium
(LMG Medium 204)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Special peptone...........................................................................23.0g
Agar ............................................................................................15.0g
Glucose .........................................................................................5.0g
NaCl..............................................................................................5.0g
Soluble starch................................................................................1.0g
Cysteine hydrochloride.................................................................0.3g
Sheep blood, sterile defibrinated .............................................50.0mL
pH 7.1 ± 0.2 at 25°C
Source:  Special peptone is available from Oxoid Unipath.
Preparation of Medium: Add components, except sheep blood, to
950.0mL distilled/deionized water and bring volume to 1.0L. Mix thor-
oughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 45°–50°C. Aseptically add 50.0mL sterile
sheep blood. Mix thoroughly. Pour into sterile Petri dishes or distribute
into sterile tubes.
Use: For the cultivation and maintenance of Moraxella osloensis,
Moraxella atlantae, and Cellulomonas hominis.