Modified Semisolid Rappaport Vassiliadis Medium (MSRV Medium)

[所属分类:培养基配方] [发布时间:2021-6-4] [发布人:网站管理员2] [阅读次数:] [返回]

Modified Semisolid Rappaport Vassiliadis Medium
(MSRV Medium)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

MgCl 2 , anhydrous.....................................................................10.93g
NaCl............................................................................................7.34g
Casein hydrolysate......................................................................4.59g
Tryptose......................................................................................4.59g
Agar..............................................................................................2.7g
KH 2 PO 4 .......................................................................................1.47g
Malachite Green oxalate...........................................................0.037g
Novobiocin solution.................................................................10.0mL
pH 5.2 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from Oxoid
Unipath.
Novobiocin Solution:
Composition per 10.0mL:
Novobiocin .................................................................................0.02g
Preparation of Novobiocin Solution: Add novobiocin to 10.0mL
of distilled/deionized water. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except novobiocin so-
lution, to distilled/deionized water and bring volume to 990.0mL. Mix
thoroughly. Gently heat to boiling. Do not autoclave. Cool to 45°–
50°C. Aseptically add 10.0mL of sterile novobiocin solution. Mix thor-
oughly. Pour into sterile Petri dishes. Air-dry plates for at least 1 hr.
Use: For the isolation and cultivation of motile Salmonella species
from food and environmental samples.