Modified Fungal Agar Base (Modified Inhibitory Mold Agar)

[所属分类:培养基配方] [发布时间:2021-6-3] [发布人:网站管理员2] [阅读次数:] [返回]

Modified Fungal Agar Base
(Modified Inhibitory Mold Agar)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Glucose.......................................................................................20.0g
Agar............................................................................................15.0g
Casein enzymic hydrolysate.........................................................2.5g
Peptic digest of animal tissue .......................................................2.5g
Yeast extract..................................................................................5.0g
Na 2 HPO 4 .......................................................................................3.5g
KH 2 PO 4 .........................................................................................3.4g
NH 4 Cl ...........................................................................................1.4g
NaCO 3 ...........................................................................................1.0g
Chloramphenicol...........................................................................0.1g
MgSO 4 ·7H 2 O..............................................................................0.06g
Polysorbate 80 .........................................................................20.0mL
pH 7.0 ± 0.2 at 25°C
Source: This medium is available from HiMedia.
Preparation of Medium: Add components, except polysorbate 80,
to distilled/deionized water and bring volume to 980.0mL. Mix thor-
oughly. Gently heat and bring to boiling. Add polysorbate 80. Distrib-
ute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Mix thoroughly. Pour into Petri dishes or aseptically distribute into
sterile tubes.
Use: For the detection and enumeration of molds in cosmetics and toi-
letries.