Modified AEA Sporulation Medium Base

[所属分类:培养基配方] [发布时间:2021-6-3] [发布人:网站管理员2] [阅读次数:] [返回]

Modified AEA Sporulation Medium Base

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition 1070.0mL:

Biopeptone..................................................................................10.0g
Yeast extract................................................................................10.0g
Na 2 HPO 4 .....................................................................................4.36g
Ammonium acetate.......................................................................1.5g
KH 2 PO 4 .......................................................................................0.25g
MgSO 4 ·7H 2 O................................................................................0.2g
Raffinose solution....................................................................40.0mL
Carbonate solution...................................................................10.0mL
Cobalt chloride solution...........................................................10.0mL
Sodium ascorbate solution.......................................................10.0mL
pH 7.8 ± 0.2 at 25°C
Source: This medium is available from HiMedia.
Raffinose Solution:
Composition per 40.0mL:
Raffinose.......................................................................................4.0g
Preparation of Raffinose Solution:  Add raffinose to distilled/de-
ionized water and bring volume to 40.0mL. Mix thoroughly. Filter ster-

ilize.

Carbonate Solution:
Composition per 10.0mL:
Na 2 CO 3 .........................................................................................0.7g
Preparation of Carbonate Solution:  Add Na 2 CO 3 to distilled/de-
ionized water and bring volume to 10.0mL. Mix thoroughly. Filter ster-
ilize.
Cobalt Cloride Solution:
Composition per 10.0mL:
CoCl 2 .........................................................................................0.032g
Preparation of Cobalt Chloride Solution:  Add CoCl 2 to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Filter sterilize.
Sodium Ascorbate Solution:
Composition per 10.0mL:
Sodium ascorbate........................................................................0.15g
Preparation of Sodium Ascorbate Solution:  Add sodium ascor-
bate to distilled/deionized water and bring volume to 10.0mL. Mix
thoroughly. Filter sterilize.
Preparation of Medium: Add components, except raffinose, car-
bonate, cobalt chloride, and sodium ascorbate solutions, to distilled/de-
ionized water and bring volume to 1.0L. Mix thoroughly. Distribute
15.0mL aliquots into screw capped tubes. Autoclave for 15 min at 15
psi pressure–121°C. Cool to 50°C. Add 0.6L raffinose solution and
0.2mL each of the carbonate and cobalt chloride solutions dropwise to
the medium in each of the tubes. Just before using, steam the medium
for 10 min. Cool to room temperature. Aseptically add 0.2mL of fresh-
ly prepared sodium ascorbate solution to each tube of the medium.
Use: For the early sporulation of Clostridium perfringens from foods.