Mn HiVeg Agar Base with Manganese

[所属分类:培养基配方] [发布时间:2021-6-3] [发布人:网站管理员2] [阅读次数:] [返回]

Mn HiVeg Agar Base with Manganese

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar............................................................................................12.0g
KH 2 PO 4 .........................................................................................2.0g
MnCO 3 ..........................................................................................2.0g
Plant extract..................................................................................1.0g
Fe(NH 4 ) 2 SO 4 ..............................................................................0.15g
Sodium citrate.............................................................................0.15g
Yeast extract..............................................................................0.075g
Cyanocobalamin solution ........................................................10.0mL
pH 7.0 ± 0.2 at 25°C
Cyanocobalamin Solution:
Composition per 10.0mL:
Cyanocobalamin........................................................................5.0mg
Preparation of Cyanocobalamin: Add cyanocarbalamin to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Filter sterilize.
Preparation of Medium: Add components, except cyanocarbala-
min solution, to distilled/deionized water and bring volume to
990.0mL. Mix thoroughly. Gently heat and bring to boiling. Distribute
into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Cool to 50°C. Aseptically add 10.0mL sterile cyanocarbolamin solu-
tion. Mix thoroughly. Pour into sterile Petri dishes or aseptically dis-
tribute into tubes.
Use: For the cultivation of Leptothrix spp. and detection of Leptothrix
by its ability to oxidize manganous ions.