Minimal Agar, Davis

[所属分类:培养基配方] [发布时间:2021-6-2] [发布人:网站管理员2] [阅读次数:] [返回]

Minimal Agar, Davis

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar............................................................................................15.0g
K 2 HPO 4 .........................................................................................7.0g
KH 2 PO 4 .........................................................................................2.0g
(NH 4 ) 2 SO 4 ....................................................................................1.0g
Glucose.........................................................................................1.0g
Sodium citrate...............................................................................0.5g
MgSO 4 ·7H 2 O................................................................................0.1g
pH 7.0 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Medium: Add components to cold distilled/deion-
ized water and bring volume to 1.0L. Mix thoroughly. Gently heat and
bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min
at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in
tubes.
Use: For the isolation, cultivation, and characterization of nutritional
mutants of Escherichia coli.