Minimal Agar II

[所属分类:培养基配方] [发布时间:2021-6-2] [发布人:网站管理员2] [阅读次数:] [返回]

Minimal Agar II

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................20.0g
Glucose .......................................................................................20.0g
(NH 4 ) 2 SO 4 .....................................................................................5.0g
KH 2 PO 4 .........................................................................................1.0g
MgSO 4 ·7H 2 O................................................................................0.5g
NaCl..............................................................................................0.1g
CaCl 2 ·2H 2 O...................................................................................0.1g
Inositol.......................................................................................2.0mg
KI...............................................................................................1.0mg
H 3 BO 3 ........................................................................................0.5mg
ZnSO 4 ·7H 2 O..............................................................................0.4mg
MnSO 4 ·4H 2 O.............................................................................0.4mg
Thiamine·HCl ............................................................................0.4mg
Pyroxidine·HCl..........................................................................0.4mg
Niacin.........................................................................................0.4mg
Calcium pantothenate ................................................................0.4mg
p-Aminobenzoic acid.................................................................0.2mg
Riboflavin ..................................................................................0.2mg
FeCl 3 ..........................................................................................0.2mg
Na 2 MoO 4 ·4H 2 O .........................................................................0.2mg
CuSO 4 ·5H 2 O............................................................................0.04mg
Folic acid.....................................................................................2.0μg
Biotin ..........................................................................................2.0μg
Growth supplement solution....................................................10.0mL
pH 5.5 ± 0.2 at 25°C
Growth Supplement Solution:
Composition per 10.0mL:
Adenine sulfate........................................................................20.0mg
L -Arginine·HCl ........................................................................20.0mg
L -Histidine·HCl........................................................................20.0mg
Preparation of Growth Supplement Solution:  Add compo-
nents to distilled/deionized water and bring volume to 10.0mL. Mix
thoroughly. Filter sterilize.
Preparation of Medium: Add components, except growth supple-
ment solution, to distilled/deionized water and bring volume to
990.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave
for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C. Aseptically
add 10.0mL of sterile growth supplement solution. Mix thoroughly.
Pour into sterile Petri dishes or distribute into sterile tubes.
Use:  For the cultivation of Saccharomyces cerevisiae.