Mineral Salts Agar

[所属分类:培养基配方] [发布时间:2021-6-2] [发布人:网站管理员2] [阅读次数:] [返回]

Mineral Salts Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................15.0g
NaNO 3 ...........................................................................................2.0g
K 2 HPO 4 .........................................................................................1.2g
MgSO 4 ..........................................................................................0.5g
KCl................................................................................................0.5g
KH 2 PO 4 .......................................................................................0.14g
Yeast extract................................................................................0.02g
Fe 2 (SO 4 ) 3 ·H 2 O............................................................................0.01g
pH 7.2 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.2.
Gently heat and bring to boiling. Distribute into tubes. Autoclave for
15 min at 15 psi pressure–121°C. Allow tubes to cool in a slanted po-
sition. Add a strip of sterile filter paper onto cooled slant. Inoculate or-
ganisms on filter paper.
Use:  For the cultivation and maintenance of Cytophaga aurantiaca
and Sporocytophaga myxococcoides.