Agrobacterium tumefaciens Selective Medium

[所属分类:培养基配方] [发布时间:2019-9-23] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Agrobacterium tumefaciens Selective Medium
Composition per 1020mL:
Agar ............................................................................................15.0g
Erythritol.....................................................................................3.05g
K 2 HPO 4 .......................................................................................1.04g
KH 2 PO 4 .......................................................................................0.54g
Sodium taurocholate...................................................................0.29g
MgSO 4 ·7H 2 O..............................................................................0.25g
NH 4 NO 3 ......................................................................................0.16g
Cycloheximide solution...........................................................10.0mL
Selenite solution.......................................................................10.0mL
Malachite Green (0.1% solution)...............................................5.0mL
Yeast extract (1% solution)........................................................1.0mL
Selenite Solution:
Composition per 10.0mL:
NaOH............................................................................................0.5g
Na 2 SeO 3 ·5H 2 O..............................................................................0.1g
Preparation of Selenite Solution: Add components to distilled/
deionized water and bring volume to 1.0L. Mix thoroughly. Filter ster-
ilize.
Cycloheximide Solution:
Composition per 10.0mL:
Cycloheximide............................................................................0.02g
Preparation of Cycloheximide Solution: Add cycloheximide to
distilled/deionized water and bring volume to 10.0mL. Mix thorough-
ly. Filter sterilize.
Caution:  Cycloheximide is toxic. Avoid skin contact or aerosol for-
mation and inhalation.
Preparation of Medium: Add components, except cycloheximide
solution and selenite solution, to distilled/deionized water and bring
volume to 1.0L. Mix thoroughly. Distribute 100.0mL into flasks. Gen-
tly heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 50°C. Aseptically add, per 100.0mL of medium, 1.0mL
sterile selenite solution and 1.0mL cycloheximide solution. Mix thor-
oughly. Aseptically pour into sterile Petri dishes.
Use: For the selective cultivation of Agrobacterium tumefaciens biovar 2.