Middlebrook 7H9 Broth, Supplemented

[所属分类:培养基配方] [发布时间:2021-5-31] [发布人:网站管理员2] [阅读次数:] [返回]

Middlebrook 7H9 Broth, Supplemented

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Na 2 HPO 4 .......................................................................................2.5g
KH 2 PO 4 .........................................................................................1.0g
Monosodium glutamate ................................................................0.5g
(NH 4 ) 2 SO 4 .....................................................................................0.5g
Tween™ 80...................................................................................0.5g
Sodium citrate...............................................................................0.1g
MgSO 4 ·7H 2 O..............................................................................0.05g
Ferric ammonium citrate.............................................................0.04g
Mycobactin J..............................................................................2.0mg
CuSO 4 ·5H 2 O..............................................................................1.0mg
Pyridoxine..................................................................................1.0mg
ZnSO 4 ·7H 2 O..............................................................................1.0mg
Biotin .........................................................................................0.5mg
CaCl 2 ·2H 2 O................................................................................0.5mg
Dubos oleic albumin complex ...............................................100.0mL
Glycerol .....................................................................................2.0mL
pH 6.6 ± 0.2 at 25°C
Source:  Mycobactin J is available from Allied Laboratories, Inc.
Dubos Oleic Albumin Complex:
Composition per 100.0mL:
Bovine serum albumin, fraction V................................................5.0g
Oleic acid, sodium salt................................................................0.05g
NaCl (0.85% solution)...........................................................100.0mL
Preparation of Dubos Oleic Albumin Complex: Add bovine serum
albumin and oleic acid to 100.0mL of NaCl solution. Mix thoroughly.
Filter sterilize.
Preparation of Medium: Add components, except Dubos oleic al-
bumin complex, to distilled/deionized water and bring volume to
900.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave
for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically
add sterile Dubos oleic albumin complex. Mix thoroughly. Pour into
sterile Petri dishes or distribute into sterile tubes.
Use:  For the cultivation and maintenance of Mycobacterium avium.