Middlebrook 7H9 Broth with Middlebrook ADC Enrichment

[所属分类:培养基配方] [发布时间:2021-5-31] [发布人:网站管理员2] [阅读次数:] [返回]

Middlebrook 7H9 Broth with
Middlebrook ADC Enrichment

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Na 2 HPO 4 .......................................................................................2.5g
KH 2 PO 4 .........................................................................................1.0g
Monosodium glutamate ................................................................0.5g
(NH 4 ) 2 SO 4 .....................................................................................0.5g
Sodium citrate...............................................................................0.1g
MgSO 4 ·7H 2 O..............................................................................0.05g
Ferric ammonium citrate.............................................................0.04g
CuSO 4 ·5H 2 O..............................................................................1.0mg
Pyridoxine..................................................................................1.0mg
ZnSO 4 ·7H 2 O..............................................................................1.0mg
Biotin .........................................................................................0.5mg
CaCl 2 ·2H 2 O................................................................................0.5mg
Middlebrook ADC enrichment..............................................100.0mL
Glycerol .....................................................................................2.0mL
pH 6.6 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Middlebrook ADC Enrichment:
Composition per 100.0mL:
Bovine albumin fraction V ...........................................................5.0g
Glucose .........................................................................................2.0g
Catalase......................................................................................3.0mg
Source:  This enrichment is available as a prepared enrichment from
BD Diagnostic Systems.
Preparation of Middlebrook ADC Enrichment: Add compo-
nents to distilled/deionized water and bring volume to 100.0mL. Mix
thoroughly. Filter sterilize.
Preparation of Medium: Add glycerol to 900.0mL of distilled/de-
ionized water and add remaining components, except Middlebrook
ADC enrichment. Mix thoroughly. Gently heat and bring to boiling.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C.
Aseptically add 100.0mL of sterile Middlebrook ADC enrichment.
Mix thoroughly. Distribute into sterile tubes or flasks.
Use: For the isolation, cultivation, and maintenance of Mycobacte-
rium species, including Mycobacterium tuberculosis. Also used for
determining the antimicrobial susceptibility of mycobacteria.