Middlebrook 13A Medium

[所属分类:培养基配方] [发布时间:2021-5-31] [发布人:网站管理员2] [阅读次数:] [返回]

Middlebrook 13A Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 112.5mL:

Casein hydrolysate........................................................................0.1g
Tween™ 80.................................................................................0.02g
Sodium polyanetholesulfonate..................................................0.025g
Middlebrook 7H9 broth.........................................................100.0mL
Middlebrook 13A enrichment..................................................12.5mL
Catalase.................................................................................. 36,000U
14 C-substrate............................................................125μCi (185kBq)
pH 6.6 ± 0.2 at 25°C
Middlebrook 7H9 Broth:
Composition per liter:
Na 2 HPO 4 .......................................................................................2.5g
KH 2 PO 4 .........................................................................................1.0g
Monosodium glutamate................................................................0.5g
(NH 4 ) 2 SO 4 ....................................................................................0.5g
Sodium citrate...............................................................................0.1g
MgSO 4 ·7H 2 O..............................................................................0.05g
Ferric ammonium citrate.............................................................0.04g
CuSO 4 ·5H 2 O..............................................................................1.0mg
Pyridoxine..................................................................................1.0mg
ZnSO 4 ·7H 2 O..............................................................................1.0mg
Biotin.........................................................................................0.5mg
CaCl 2 ·2H 2 O ...............................................................................0.5mg
Glycerol .....................................................................................2.0mL
Preparation of Middlebrook 7H9 Broth:  Add components to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Middlebrook 13A Enrichment:
Composition per 20.0mL:
Bovine serum albumin..................................................................3.0g
Preparation of Middlebrook 13A Enrichment:  Add bovine se-
rum albumin to distilled/deionized water and bring volume to 20.0mL.
Mix thoroughly. Filter sterilize.
Preparation of Medium:  To 100.0mL of Middlebrook 7H9 broth,
add remaining components, except Middlebrook 13A enrichment. Mix
thoroughly. Filter sterilize. Aseptically distribute into bottles in 4.0mL
volumes. Prior to inoculation, aseptically add 0.5mL of Middlebrook
13A enrichment to each bottle. Mix thoroughly.
Use: For the cultivation of Mycobacterium species from the blood of
patients suspected of having mycobacteremia.