Microcella alkaliphila Medium (DSMZ Medium 1063)

[所属分类:培养基配方] [发布时间:2021-5-28] [发布人:网站管理员2] [阅读次数:] [返回]

Microcella alkaliphila Medium
(DSMZ Medium 1063)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein..........................................................17.0g
NaCl..............................................................................................5.0g
Papaic digest of soybean meal......................................................3.0g
Yeast extract..................................................................................3.0g
K 2 HPO 4 .........................................................................................2.5g
Glucose .........................................................................................2.5g
Na-sesquicarbonate solution..................................................100.0mL
Mineral salt solution ................................................................50.0mL
Vitamin solution.........................................................................5.0mL
Trace elements solution SL-10 ..................................................1.0mL
pH 9.5 ± 0.2 at 25°C
Sodium Sesquicarbonate Solution:
Composition per 100.0mL:
Na 2 CO 3 , anhydrous.....................................................................10.6g
NaHCO 3 ......................................................................................8.42g
Preparation of Sodium Sesquicarbonate Solution : Add com-
ponents to distilled/deionized water and bring volume to 100.0mL.
Mix thoroughly. Filter sterilize.
Mineral Solution:
Composition per liter:
KH 2 PO 4 .......................................................................................10.0g
MgCl 2 ·6H 2 O..................................................................................6.6g
NaCl..............................................................................................8.0g
NH 4 Cl ...........................................................................................8.0g
CaCl 2 ·2H 2 O...................................................................................1.0g
Preparation of Mineral Solution: Add components to distilled/
deionized water and bring volume to 1.0L. Mix thoroughly.
Vitamin Solution:
Composition per liter:
Pyridoxine·HCl..........................................................................6.2mg
Nicotinic acid.............................................................................2.5mg
Thiamine·HCl ..........................................................................1.25mg
p-Aminobenzoic acid...............................................................1.25mg
Pantothenic acid.......................................................................0.62mg
Biotin .......................................................................................0.25mg
Preparation of Vitamin Solution : Add components to distilled/
deionized water and bring volume to 1.0L. Mix thoroughly.
Trace Elements Solution SL-10:
Composition per liter:
MgSO 4 ·7H 2 O................................................................................3.0g
Nitrilotriacetic acid.......................................................................1.5g
NaCl..............................................................................................1.0g
MnSO 4 ·2H 2 O................................................................................0.5g
CoSO 4 ·7H 2 O...............................................................................0.18g
ZnSO 4 ·7H 2 O...............................................................................0.18g
CaCl 2 ·2H 2 O ..................................................................................0.1g
FeSO 4 ·7H 2 O..................................................................................0.1g
NiCl 2 ·6H 2 O...............................................................................0.025g
KAl(SO 4 ) 2 ·12H 2 O.......................................................................0.02g
H 3 BO 3 .........................................................................................0.01g
Na 2 MoO 4 ·4H 2 O..........................................................................0.01g
CuSO 4 ·5H 2 O...............................................................................0.01g
Na 2 SeO 3 ·5H 2 O...........................................................................0.3mg
Preparation of Trace Elements Solution SL-10:  Add nitrilotri-
acetic acid to 500.0mL of distilled/deionized water. Dissolve by adjust-
ing pH to 6.5 with KOH. Add remaining components. Add distilled/
deionized water to 1.0L. Mix thoroughly. Adjust pH to 7.0.
Preparation of Medium: Add components, except sodium sesqui-
carbonate solution, to distilled/deionized water and bring volume to
900.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to room temperature. Aseptically add sterile 1M sodium
sesquicarbonate solution to achieve a final pH of 9.5. Aseptically dis-
pense into tubes, flasks, or bottles.
Use:  For the cultivation of Microcella alkaliphila.