Micro Assay Culture Agar

[所属分类:培养基配方] [发布时间:2021-5-28] [发布人:网站管理员2] [阅读次数:] [返回]

Micro Assay Culture Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Yeast extract................................................................................20.0g
Agar............................................................................................10.0g
Glucose.......................................................................................10.0g
Proteose peptone No. 3.................................................................5.0g
KH 2 PO 4 .........................................................................................2.0g
Sorbitan monooleate complex ......................................................0.1g
pH 6.7 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes in 10.0mL volumes. Autoclave for 15
min at 15 psi pressure–121°C. Just prior to solidification of the agar,
disperse precipitate by gently twirling tube.
Use: For carrying stock cultures of lactobacilli and other test microor-
ganisms used in microbiological assays. For the general cultivation of
lactobacilli.