MH Salts

[所属分类:培养基配方] [发布时间:2021-5-28] [发布人:网站管理员2] [阅读次数:] [返回]

MH Salts

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NaCl..........................................................................................120.5g
MgCl 2 ·6H 2 O................................................................................22.4g
Agar ............................................................................................20.0g
MgSO 4 ........................................................................................14.4g
Yeast extract................................................................................10.0g
Proteose peptone No. 3.................................................................5.0g
KCl................................................................................................3.0g
Glucose .........................................................................................1.0g
CaCl 2 ...........................................................................................0.54g
NaHCO 3 ......................................................................................0.09g
NaBr..........................................................................................0.039g
pH 7.5 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.5.
Gently heat and bring to boiling. Distribute into tubes or flasks. Auto-
clave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes
or leave in tubes.
Use: For the cultivation and maintenance of Bacillus halophilus.