MH IH Agar

[所属分类:培养基配方] [发布时间:2021-5-28] [发布人:网站管理员2] [阅读次数:] [返回]

MH IH Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Solution A..............................................................................490.0mL
Solution B..............................................................................490.0mL
Supplement solution ................................................................20.0mL
pH 6.9 ± 0.2 at 25°C
Solution A:
Composition per 490.0mL:
Beef infusion.............................................................................300.0g
Acid hydrolysate of casein..........................................................17.5g
Agar............................................................................................17.0g
Starch............................................................................................1.5g
Preparation of Solution A: Add components to distilled/deionized
water and bring volume to 490.0mL. Mix thoroughly. Gently heat and
bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool
to 45°–50°C.
Solution B:
Composition per 490.0mL:
Hemoglobin................................................................................10.0g
Preparation of Solution B: Add hemoglobin to distilled/deionized
water and bring volume to 490.0mL. Mix thoroughly. Gently heat and
bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool
to 45°–50°C.
Supplement Solution:
Composition per liter:
Glucose.....................................................................................100.0g
L- Cysteine·HCl............................................................................25.9g
L- Glutamine ................................................................................10.0g
L- Cystine.......................................................................................1.1g
Adenine.........................................................................................1.0g
Nicotinamide adenine dinucleotide ............................................0.25g
Vitamin B 12 ...................................................................................0.1g
Thiamine pyrophosphate ..............................................................0.1g
Guanine·HCl...............................................................................0.03g
Fe(NO 3 ) 3 ·6H 2 O...........................................................................0.02g
p-Aminobenzoic acid................................................................0.013g
Thiamine·HCl ............................................................................3.0mg
Source: The supplement solution IsoVitaleX ® enrichment is available
from BD Diagnostic Systems. This enrichment may be replaced by
supplement VX from BD Diagnostic Systems.
Preparation of Supplement Solution: Add components to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly. Filter
sterilize.
Preparation of Medium:  Aseptically combine cooled, sterile solu-
tion A and cooled, sterile solution B. Mix thoroughly. Adjust pH to 6.9
with sterile 1N HCl or sterile 1N KOH. Aseptically add 20.0mL of ster-
ile supplement solution. Pour into sterile Petri dishes or distribute into
sterile tubes.
Use: For the cultivation and differentiation of Legionella species.