Agrobacterium Agar with Biotin

[所属分类:培养基配方] [发布时间:2019-9-23] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Agrobacterium Agar with Biotin
Composition per liter:
Agar ............................................................................................15.0g
Mannitol........................................................................................8.0g
NaCl..............................................................................................5.0g
Yeast extract..................................................................................5.0g
(NH 4 ) 2 SO 4 ....................................................................................2.0g
Casamino acids.............................................................................0.5g
Biotin solution............................................................................0.1mL
pH 6.6 ± 0.2 at 25°C
Biotin Solution:
Composition per 10.0mL:
Biotin .........................................................................................0.2mg
Preparation of Biotin Solution:  Add biotin to distilled/deionized
water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except biotin solution,
to distilled/deionized water and bring volume to 999.9mL. Mix thor-
oughly. Gently heat and bring to boiling. Adjust pH to 6.6. Autoclave
for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C. Aseptically
add 0.1mL of sterile biotin solution. Mix thoroughly. Pour into sterile
Petri dishes or distribute into sterile tubes.
Use:  For the cultivation and maintenance of slow-growing Agrobac-
terium rhizogenes and Agrobacterium tumefaciens.