Methylotrophic Arthrobacter Hyphomicrobium Medium (DSMZ Medium 939)

[所属分类:培养基配方] [发布时间:2021-5-27] [发布人:网站管理员2] [阅读次数:] [返回]

Methylotrophic Arthrobacter Hyphomicrobium Medium
(DSMZ Medium 939)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Na 2 HPO 4 ·2H 2 O.............................................................................7.9g
Dimethylsulfone............................................................................1.9g
KH 2 PO 4 .........................................................................................1.5g
NH 4 Cl ...........................................................................................0.8g
MgSO 4 ·7H 2 O................................................................................0.1g
Trace elements solution ...........................................................10.0mL
pH 7.2-7.5 at 25°C
Trace Elements Solution:
Composition per liter:
EDTA disodium salt....................................................................50.0g
NaOH............................................................................................9.0g
CaCl 2 ·2H 2 O.................................................................................7.34g
FeSO 4 ·7H 2 O..................................................................................5.0g
MnCl 2 ·4H 2 O..................................................................................2.5g
ZnSO 4 ·7H 2 O.................................................................................1.0g
CoCl 2 ·6H 2 O..................................................................................0.5g
NH 4 (MoO 4 )...................................................................................0.5g
CuSO 4 ·5H 2 O.................................................................................0.2g
Preparation of Trace Elements Solution: Add Na 2 -EDTA to
400.0mL distilled/deionized water. Mix thoroughly. Add 9.0g NaOH.
Mix thoroughly. Individually dissolve each of the other components in
40.0mL distilled/deionized water. Add each of the other dissolved
components to the EDTA solution and bring volume to 1.0L with dis-
tilled/deionized water. Mix thoroughly. Adjust the pH to 6.0 with 1M
NaOH.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes
or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the cultivation of Hyphomicrobium sulfonivorans.