Methylophaga sulfidovorans Medium (DSMZ Medium 951)

[所属分类:培养基配方] [发布时间:2021-5-27] [发布人:网站管理员2] [阅读次数:] [返回]

Methylophaga sulfidovorans Medium
(DSMZ Medium 951)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NaCl............................................................................................15.0g
Na 2 CO 3  .........................................................................................2.0g
MgSO 4 ·7H 2 O................................................................................1.0g
(NH 4 ) 2 SO 4 .....................................................................................0.5g
CaCl 2 ·6H 2 O.................................................................................0.33g
KCl................................................................................................0.2g
KH 2 PO 4 .......................................................................................0.02g
DMS (Dimethylsulphide) ........................................................62.0mg
FeSO 4 ·7H 2 O...............................................................................1.0mg
Trace elements solution SL-10 ..................................................1.0mL
Vitamin solution.........................................................................1.0mL
pH 7.5 ± 0.3 at 25°C
Trace Elements Solution SL-10:
Composition per liter:
FeCl 2 ·4H 2 O...................................................................................1.5g
CoCl 2 ·6H 2 O ...........................................................................190.0mg
MnCl 2 ·4H 2 O...........................................................................100.0mg
ZnCl 2 ........................................................................................70.0mg
Na 2 MoO 4 ·2H 2 O .......................................................................36.0mg
NiCl 2 ·6H 2 O..............................................................................24.0mg
H 3 BO 3 ........................................................................................6.0mg
CuCl 2 ·2H 2 O ...............................................................................2.0mg
HCl (25% solution)..................................................................10.0mL
Preparation of Trace Elements Solution SL-10: Add FeCl 2 ·4H 2 O
to 10.0mL of HCl solution. Mix thoroughly. Add distilled/deionized
water and bring volume to 1.0L. Add remaining components. Mix thor-
oughly. Sparge with 80% N 2 + 20% CO 2 .
Vitamin Solution:
Composition per liter:
Pyridoxine-HCl......................................................................500.0mg
Nicotinic acid.........................................................................200.0mg
Thiamine................................................................................100.0mg
p-Aminobenzoic acid.............................................................100.0mg
Pantothenate.............................................................................50.0mg
Biotin.......................................................................................20.0mg
Riboflavin................................................................................10.0mg
Vitamin B 12 ..............................................................................10.0mg
Preparation of Vitamin Solution:  Add components to distilled/
deionized water and bring volume to 1.0L. Mix thoroughly. Sparge
with 80% H 2 + 20% CO 2 .
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.5.
Distribute into tubes or bottles. Autoclave for 15 min at 15 psi pres-
sure–121°C.
Use: For the cultivation of Methylophaga sulfidovorans.