Methylophaga alcalica Agar (DSMZ Medium 976)

[所属分类:培养基配方] [发布时间:2021-5-27] [发布人:网站管理员2] [阅读次数:] [返回]

Methylophaga alcalica Agar
(DSMZ Medium 976)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NaCl............................................................................................30.0g
Agar............................................................................................20.0g
KH 2 PO 4 .........................................................................................1.0g
KNO 3 ............................................................................................1.0g
MgSO 4 ·7H 2 O..............................................................................0.22g
Na 2 CO 3  solution......................................................................50.0mL
Methanol solution....................................................................50.0mL
Trace elements solution.............................................................1.0mL
pH 9.5 ± 0.2 at 25°C
Methanol Solution:
Composition per 50.0mL:
Methanol..................................................................................10.0mL
Preparation of Methanol Solution: Add methanol to distilled/de-
ionized water and bring volume to 50.0mL. Mix thoroughly. Autoclave
for 15 min at 15 psi pressure–121°C.
Na 2 CO 3 Solution:
Composition per 50.0mL:
Na 2 CO 3  .........................................................................................5.0g
Preparation of Na 2 CO 3 Solution: Add Na 2 CO 3  to distilled/de-
ionized water and bring volume to 50.0mL. Mix thoroughly. Autoclave
for 15 min at 15 psi pressure–121°C.
Trace Elements Solution:
Composition per liter:
Ferric citrate.............................................................................30.0mg
CaCl 2 ·2H 2 O..............................................................................30.0mg
MgCl 2 ·4H 2 O...............................................................................5.0mg
ZnSO 4 ·7H 2 O..............................................................................5.0mg
CuSO 4 ·5H 2 O..............................................................................0.5mg
Preparation of Trace Elements Solution: Add components to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Add components, except methanol solu-
tion and Na 2 CO 3  solution, to distilled/deionized water and bring vol-
ume to 900.0mL. Mix thoroughly. Gently heat and bring to boiling.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 55°C. Asepti-
cally add 50.0mL warm sterile Na 2 CO 3  solution and 50.0mL warm
sterile methanol solution. Mix thoroughly. Pour into Petri dishes or
aseptically distribute into sterile tubes.
Use: For the cultivation and maintenance of Methylophaga alcalica.