Methylomicrobium Medium (DSMZ Medium 1180)

[所属分类:培养基配方] [发布时间:2021-5-27] [发布人:网站管理员2] [阅读次数:] [返回]

Methylomicrobium Medium
(DSMZ Medium 1180)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NaCl ...........................................................................................30.0g
MgSO 4 · 7H 2 O................................................................................0.2g
CaCl 2 ·2H 2 O ................................................................................0.02g
KNO 3 ............................................................................................1.0g
Bicarbonate solution................................................................50.0mL
Phosphate buffer solution........................................................20.0mL
Carbonate solution.....................................................................5.0mL
Trace elements solution.............................................................1.0mL
pH 8.7 ± 0.2 at 25°C
Phosphate Buffer Solution:
Composition per liter:
Na 2 HPO 4 ·12H 2 O.........................................................................30.0g
KH 2 PO 4 .......................................................................................14.0g
Preparation of Phosphate Buffer Solution:  Add components to
distilled/deionized water and bring volume to 1.0L. Filter sterilize
Bicarbonate Solution :
Composition per 100.0mL:
NaHCO 3 ........................................................................................7.2g
Preparation of Bicarbonate Solution:  Add NaHCO 3 to distilled/
deionized water and bring volume to 100.0mL. Mix thoroughly. Auto-
clave for 15 min at 15 psi pressure–121°C. Cool to room temperature.
Carbonate Solution :
Composition per 10.0mL:
Na 2 CO 3 .........................................................................................1.1g
Preparation of Carbonate Solution:  Add Na 2 CO 3 to distilled/de-
ionized water and bring volume to 10.0mL. Mix thoroughly. Autoclave
for 15 min at 15 psi pressure–121°C. Cool to room temperature.
Trace Elements Solution:
Composition per liter:
EDTA............................................................................................5.0g
FeSO 4 ·7H 2 O................................................................................. 2.0g
CoCl 2 ·6H 2 O..................................................................................0.2g
CuCl 2 ·2H 2 O..................................................................................0.1g
ZnSO 4 ·7H 2 O................................................................................ 0.1g
MnCl 2 ·4H 2 O ...............................................................................0.03g
H 3 BO 3 .........................................................................................0.03g
Na 2 MoO 4 ·2H 2 O......................................................................... 0.03g
NiCl 2 ·6H 2 O.................................................................................0.02g
Preparation of Trace Elements Solution:  Add components to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly. Au-
toclave for 15 min at 15 psi pressure–121°C.
Preparation of Medium: Add components, except carbonate, bicar-
bonate, and phosphate buffer solutions, to distilled/deionized water and
bring volume to 925.0mL. Mix thoroughly. Autoclave for 15 min at 15
psi pressure–121°C. Cool to room temperature. Aseptically add the car-
bonate, bicarbonate, and phosphate buffer solutions. Aseptically dis-
tribute into culture vessels.
Use: For the cultivation with faster growth rates of Methylomicrobium
spp.