Agar Medium P (PM Indicator Agar)

[所属分类:培养基配方] [发布时间:2019-9-23] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Agar Medium P
(PM Indicator Agar)
Composition per liter:
Agar............................................................................................15.0g
Glucose.......................................................................................5.25g
Peptone .........................................................................................5.0g
Beef extract...................................................................................3.0g
Pancreatic digest of casein............................................................1.7g
Tween™ 80...................................................................................1.0g
NaCl..............................................................................................0.5g
Papaic digest of soybean meal......................................................0.3g
K 2 HPO 4 ......................................................................................0.25g
Bromcresol Purple......................................................................0.06g
pH 7.8 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation of Bacillus stearothermophilus for the detec-
tion of penicillin in milk.