Methanothermus fervidus Medium

[所属分类:培养基配方] [发布时间:2021-5-27] [发布人:网站管理员2] [阅读次数:] [返回]

Methanothermus fervidus Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Na 2 SO 4 ..........................................................................................3.4g
NaHCO 3 ........................................................................................2.0g
L -Cysteine·HCl .............................................................................0.5g
Na 2 S·9H 2 O....................................................................................0.5g
FeSO 4 ·7H 2 O...............................................................................2.0mg
Ni(NH 4 ) 2 (SO 4 ) 2 .........................................................................2.0mg
Resazurin...................................................................................1.0mg
Mineral solution 1....................................................................37.5mL
Mineral solution 2....................................................................37.5mL
Trace elements solution...........................................................10.0mL
pH 6.5 ± 0.2 at 25°C
Mineral Solution 1:
Composition per liter:
K 2 HPO 4 .........................................................................................6.0g
Preparation of Mineral Solution 1 : Add K 2 HPO 4 to distilled/de-
ionized water and bring volume to 1.0L. Mix thoroughly.
Mineral Solution 2:
Composition per liter:
NaCl............................................................................................12.0g
K 2 HPO 4 .........................................................................................6.0g
(NH 4 ) 2 SO 4 .....................................................................................6.0g
MgSO 4 ·7H 2 O................................................................................2.4g
CaCl 2 ·2H 2 O...................................................................................1.6g
Preparation of Mineral Solution 2 : Add components to distilled/
deionized water and bring volume to 1.0L. Mix thoroughly.
Trace Elements Solution:
Composition per liter:
MgSO 4 ·7H 2 O............................................................................... 3.0g
Nitrilotriacetic acid.......................................................................1.5g
NaCl..............................................................................................1.0g
MnSO 4 ·2H 2 O............................................................................... 0.5g
CoSO 4 ·7H 2 O...............................................................................0.18g
ZnSO 4 ·7H 2 O...............................................................................0.18g
CaCl 2 ·2H 2 O...................................................................................0.1g
FeSO 4 ·7H 2 O..................................................................................0.1g
NiCl 2 ·6H 2 O...............................................................................0.025g
KAl(SO 4 ) 2 ·12H 2 O.......................................................................0.02g
CuSO 4 ·5H 2 O...............................................................................0.01g
H 3 BO 3 .........................................................................................0.01g
Na 2 MoO 4 ·2H 2 O ......................................................................... 0.01g
Na 2 SeO 3 ·5H 2 O...........................................................................0.3mg
Preparation of Trace Elements Solution:  Add nitrilotriacetic
acid to approximately 500.0mL of distilled/deionized water. Dissolve
by adding KOH and adjust pH to 6.5. Add remaining components.
Bring volume to 1.0L with additional distilled/deionized water. Adjust
pH to 7.0 with KOH.
Preparation of Medium: Add components, except NaHCO 3 and
Na 2 S·9H 2 O, to distilled/deionized water and bring volume to 1.0L. Ad-
just pH to 6.5 with 10N H 2 SO 4 . Add NaHCO 3  and sparge with 80% N 2
+ 20% CO 2  for 15 min. Add Na 2 S·9H 2 O. Mix thoroughly. Anaerobical-
ly distribute 20.0mL of medium into 100mL alkali-rich soda lime glass
bottles. Pressurize to 2 bar with 80% H 2 + 20% CO 2 . Autoclave for 15
min at 15 psi pressure–121°C.
Use: For the cultivation and maintenance of Methanothermus sociabi-
lis.